1. The intensity of each band detected by the anti-H3K36Me antibodies was normalized to that of the anti-H3 antibody to compare each modification level. Analysis of transcript accumulation by RNA-seq and qRT-PCR Each P. ostreatus strain was grown on BWS-I for 13 and 20 d. Total RNA was isolated using the FastGene RNA Premium Kit (NIPPON Genetics;Cambridge Abcam;histone H3,1791
2. was used to measure the concentration of the reverse transcript (single-stranded DNA) for normalization of the qRT-PCR results under this culture condition. The fluorescence of each sample was measured using a Qubit 4 fluorometer (Thermo Fisher), and green emission excited by blue light (470 nm) was measured at 510-580 nm. The concentration was calculated based on a standard curve generated Reference;Thermo Fisher;The Quant-iT OliGreen ssDNA Assay Kit,2020
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