Author:
Pereira Cátia D.,Espadas Guadalupe,Martins Filipa,Bertrand Anne T.,Servais Laurent,Sabidó Eduard,Chevalier Philippe,Da Cruz e Silva Odete A. B.,Rebelo Sandra
Reference124 articles.
1. Germany) to test their susceptibility to DNA damage or oxidative stress, respectively. The two cell lines were initially seeded at specific densities and grown in standard conditions for 48 h to achieve a similar confluency of 80-90% before exposure to the stress agents. Subsequently, cells were incubated at 37 �C in fresh culture medium with different concentrations of bleomycin or H 2 O 2 during specific time points. A control condition in which no bleomycin or H 2 O 2 was added to the culture medium was also included in each experiment;Merck Sigma-Aldrich;After cell treatments