Abstract
Objective: This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 (<i>DUSP8</i>) and insulin-like growth factor 2 (<i>IGF2</i>) genes and to explore their effects on inosine-5′-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line).Methods: A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of <i>DUSP8</i> and <i>IGF2</i> genes, respectively. One SNP (rs313443014 C>T) in <i>DUSP8</i> gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens.Results: The DUSP8 (rs313443014 C>T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The <i>IGF2</i> gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p<0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p<0.05) on nucleotide content was also observed.Conclusion: The SNPs in the <i>DUSP8</i> and <i>IGF2</i> genes might be used as genetic markers in the selection and production of chickens with highly flavored meat.
Funder
Rural Development Administration
Publisher
Asian Australasian Association of Animal Production Societies
Subject
General Veterinary,Genetics,Animal Science and Zoology,Physiology,Food Science