Comparison of rectum fecal bacterial community of finishing bulls fed high-concentrate diets with active dry yeast and yeast culture supplementation

Abstract

Objective: The objective of this study was to investigate the effects of feeding active dry yeast (ADY) and yeast culture (YC) on fecal bacterial community in finishing bulls fed high-concentrate diets in the same experimental environment.Methods: Forty-five healthy finishing cattle (Simmental×Chinese Luxi yellow bulls; 24 months; 505±29 kg) were randomly divided into three groups: i) CON group (control group, only fed basal diet), ii) ADY group (fed basal diet + active dry yeast), and iii) YC group (fed basal diet + yeast culture). At the end of the trial, nine rectum fecal samples were randomly selected from each group for bacterial DNA sequencing.Results: There was no difference among groups about alpha diversity indices (all p>0.05), including ACE, Chao 1, Shannon, and Simpson indices. Principal component analysis and non-metric multidimensional scaling analysis showed a high similarity among three groups. Compared with CON group, ADY and YC groups had greater relative abundance of c_<i>Clostridia</i>, o_<i>Oscillospirales</i>, and f_<i>Oscillospiraceae</i>, but lesser relative abundance of g_<i>Megasphaera</i>, and s_<i>Megasphaera</i>_<i>elsdenii</i> (all p<0.01). And, the relative abundances of p_<i>Firmicutes</i> (p = 0.03), s_<i>Prevotella</i>_<i>sp</i> (p = 0.03), o_<i>Clostridiales</i> (p<0.01), g_<i>Clostridium</i> (p<0.01), f_<i>Caloramatoraceae</i> (p<0.01), and f_<i>Ruminococcaceae</i> (p = 0.04) were increased in the ADY group. The PICRUSt2 prediction results showed that the metabolic pathways had no significant differences among groups (p>0.05). Besides, the relative abundance of c_<i>Clostridia</i> (r = 0.42), and f_<i>Oscillospiraceae</i> (r = 0.40) were positively correlated to average daily gain of finishing bulls (p<0.05).Conclusion: Both of ADY and YC had no effect on diversity of fecal bacteria in finishing bulls, but the supplementation of ADY and YC can improve the large intestinal function in finishing bulls by increasing the abundance of cellulolytic bacteria and altering the abundance of lactic acid-utilizing bacteria.