Influence of tail versus cardiac sampling on blood glucose and lipid profiles in mice

Author:

Chan Yih Kai1,Davis Paul F23,Poppitt Sally D14,Sun Xueying5,Greenhill Nicholas S23,Krishnamurthi Rita5,Przepiorski Aneta5,McGill Anne-Thea16,Krissansen Geoffrey W5

Affiliation:

1. Human Nutrition Unit, The University of Auckland, Auckland, New Zealand

2. Bioactivity Investigation Group, Department of Medicine, University of Otago, Wellington, Wellington South, New Zealand

3. Trinity Bioactives Ltd, Wellington, New Zealand

4. School of Biological Sciences, The University of Auckland, Auckland, New Zealand

5. Department of Molecular Medicine & Pathology, Faculty of Medical and Health Sciences, University of Auckland, New Zealand

6. School of Population Health, The University of Auckland, Auckland, New Zealand

Abstract

Blood is collected during animal experimentation to measure haematological and metabolic parameters. It cannot be assumed that circulating blood has the same composition irrespective of its location, and indeed, differences in the composition of blood sampled from the arterial and venous compartments have been reported. Here we investigated whether blood collected by cardiac puncture (CP) versus that collected following removal of the distal 1 mm of the tail tip (TT) differs with respect to glucose and lipid profiles in male C57BL/6J mice at 4, 7, 20 and 28 weeks of age. Blood was first collected from the TT of unanaesthetized mice, which were then immediately anaesthetized using ketamine/xylazine, and a second blood sample was collected by CP. The CP glucose concentration was significantly higher than TT glucose by a positive bias averaging +80% ( P < 0.01), irrespective of the age of the mice. Conversely, the concentrations of the CP lipids, including total cholesterol, high-density lipoprotein cholesterol and triglyceride were lower than TT lipids by a negative bias averaging −25% ( P < 0.05). These observations highlight the difficulty in measuring and comparing metabolic parameters such as glucose and lipid between one blood compartment and another. They illustrate the need to standardize sampling sites, especially when repeated blood sampling is required.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

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