BLCAP induces apoptosis in human Ewing's sarcoma cells-Reference-Cited by-同舟云学术

BLCAP induces apoptosis in human Ewing's sarcoma cells

Published:2011-09 Issue:9 Volume:236 Page:1030-1035
ISSN:1535-3702
Container-title:Experimental Biology and Medicine
language:en
Short-container-title:Exp Biol Med (Maywood)

Author:

Fan De-Gang¹,Zhao Feng²,Ding Yong¹,Wu Ming-Mei³,Fan Qing-Yu¹,Shimizu Katsuji⁴,Dohjima Taikoh⁴,Nozawa Satoshi⁴,Wakahara Kazuhiko⁴,Ohno Takatoshi⁴,Guo Yun-Shan⁵,Ma Bao-An¹,Jiang Jian-Li⁵

Affiliation:

1. Orthopaedic Oncology Institute of Chinese PLA, Tangdu Hospital

2. Department of Respiratory Disease, Xijing Hospital, The Fourth Military Medical University, Xi'an 710038

3. Institute of Neuroscience, The Fourth Military Medical University, Xi'an 710032, China

4. Department of Orthopaedic Surgery, Gifu University School of Medicine, Yanagido 1-1, Gifu 501-1194, Japan

5. Cell Engineering Research Centre and Department of Cell Biology, The Fourth Military Medical University, Xi'an 710032, China

Abstract

Bladder cancer-associated protein (BLCAP) is a novel candidate tumor suppressor gene identified from human bladder carcinoma and highly associated with the invasion of bladder cancer. We previously reported that it also plays a key role in the tumorigenesis and metastasis of human osteosarcoma. In the present study, we constructed a recombinant encoding BLCAP cDNA. Overexpression of BLCAP resulted in growth inhibition and induced apoptosis of human TC-135 Ewing's sarcoma cells in vitro. We further investigated the caspase-3/7 activity and expressions of the fusion transcription factor Ewing's sarcoma protein-friend leukemia virus integration 1 (EWS-FLI1) and the apoptosis regulator B-cell lymphoma 2 (BCL-2). Cell apoptosis was accompanied by the down-regulated expression of EWS-FLI1 and BCL-2. Our present results suggest that BLCAP may play a role not only in regulating cell proliferation but also in coordinating apoptosis through the down-regulation of BCL-2 and EWS-FLI1 in human Ewing's sarcoma cells.

Publisher

SAGE Publications

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://journals.sagepub.com/doi/pdf/10.1258/ebm.2011.010315

Reference21 articles.

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3. EWS/FLI-1 Silencing and Gene Profiling of Ewing Cells Reveal Downstream Oncogenic Pathways and a Crucial Role for Repression of Insulin-Like Growth Factor Binding Protein 3

4. EWS-Fli1 antisense oligodeoxynucleotide inhibits proliferation of human Ewing's sarcoma and primitive neuroectodermal tumor cells.

5. Small interfering RNAs expressed from a Pol III promoter suppress the EWS/Fli-1 transcript in an Ewing sarcoma cell line

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