The 'normalization' of germ-free rabbits with host-specific caecal microflora-Reference-Cited by-同舟云学术

The 'normalization' of germ-free rabbits with host-specific caecal microflora

Published:1985-10-01 Issue:4 Volume:19 Page:344-352
ISSN:0023-6772
Container-title:Laboratory Animals
language:en
Short-container-title:Lab Anim

Author:

Boot R.¹,Koopman J. P.²,Kruijt B. C.³,Lammers R. M.⁴,Kennis Henriëtte M.²,Lankhorst A.⁵,Mullink J. W. M. A.⁶,Stadhouders A. M.⁷,De Boer H.⁷,Welling G. W.⁸,Hectors M. P. C.⁹

Affiliation:

1. Department of Animal Supply, National Institute of Public Health and Environmental Hygiene, P.O. Box, 3720 BA Bitthoven, The Netherlands; Department for Laboratory Animal Bacteriology, National Institute of Public Health and Environmental Hygiene, P.O. Box, 3720 BA Bitthoven, The Netherlands

2. Central Animal Laboratory, Catholic University, Nijmegen, The Netherlands

3. Department of Animal Supply, National Institute of Public Health and Environmental Hygiene, P.O. Box, 3720 BA Bitthoven, The Netherlands

4. Department for Laboratory Animal Bacteriology, National Institute of Public Health and Environmental Hygiene, P.O. Box, 3720 BA Bitthoven, The Netherlands

5. Department for Laboratory Animal Science, Faculty of Veterinary Medicine, State University, Utrecht, The Netherlands

6. Central Institute for the Breeding of Laboratory Animals TNO Zeist, Catholic University, Nijmegen, The Netherlands

7. Department of Submicroscopical Morphology, Catholic University, Nijmegen, The Netherlands

8. Department of Medical Microbiology, State University Groningen, Nijmegen, The Netherlands

9. Department of Gastroenterology, Catholic University, Nijmegen, The Netherlands

Abstract

Hysterectomy-derived germ-free rabbits were given colonization-resistant caecal flora (CRF) from mice, or microflora obtained from the caecum of an antibiotic-decontaminated conventional rabbit and compared with rabbits conventionally raised with the doe. Bodyweight and the following intestinal parameters were determined for the 3 groups: colonization resistance to E. coli, relative caecal weight, villus:crypt ratio (ileum), β-aspartylglycine (faeces), volatile fatty acids (caecum), and bile acids (faeces). Germ-free rabbits given mouse CRF-flora showed values quite different from control animals (or most parameters, indicating unsuitability of mouse CRF flora to 'normalize' rabbits. In germ-free rabbits given modified (antibiotic-treated) rabbit flora, values for most parameters were intermediate between those found for the other 2 groups. This species-specific caecal flora should be improved to provide full 'normalization' of germ-free rabbits.

Publisher

SAGE Publications

Subject

General Veterinary,Animal Science and Zoology

Link

http://journals.sagepub.com/doi/pdf/10.1258/002367785780887428

Cited by 21 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Sending signals – The microbiota’s contribution to intestinal epithelial homeostasis;Microbes and Infection;2021-07

2. Bacterial, Mycoplasmal, and Mycotic Infections;The Laboratory Rat;2020

3. Phylosymbiosis: Relationships and Functional Effects of Microbial Communities across Host Evolutionary History;PLOS Biology;2016-11-18

4. The Mammalian Microbiome and Its Importance in Laboratory Animal Research;ILAR Journal;2015-08-31

5. Microbiological Quality Control for Laboratory Rodents and Lagomorphs;Laboratory Animal Medicine;2015