Klf5 is involved in self-renewal of mouse embryonic stem cells
Author:
Parisi Silvia12, Passaro Fabiana13, Aloia Luigi12, Manabe Ichiro4, Nagai Ryozo5, Pastore Lucio13, Russo Tommaso13
Affiliation:
1. CEINGE Biotecnologie Avanzate, 80145 Napoli, Italy 2. European School of Molecular Medicine, SEMM, 80145 Napoli, Italy 3. Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, 80131 Napoli, Italy 4. Nano-Bioengineering Education Program, The University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan 5. Department of Cardiovascular Medicine, The University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan
Abstract
Self-renewal of embryonic stem cells (ESCs) is maintained by a complex regulatory mechanism involving transcription factors Oct3/4 (Pou5f1), Nanog and Sox2. Here, we report that Klf5, a Zn-finger transcription factor of the Kruppel-like family, is involved in ESC self-renewal. Klf5 is expressed in mouse ESCs, blastocysts and primordial germ cells, and its knockdown by RNA interference alters the molecular phenotype of ESCs, thereby preventing their correct differentiation. The ability of Klf5 to maintain ESCs in the undifferentiated state is supported by the finding that differentiation of ESCs is prevented when Klf5 is constitutively expressed. Maintenance of the undifferentiated state by Klf5 is, at least in part, due to the control of Nanog and Oct3/4 transcription, because Klf5 directly binds to the promoters of these genes and regulates their transcription.
Publisher
The Company of Biologists
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