The genome of HSV-1 translocates through the nuclear pore as a condensed rod-like structure-Reference-Cited by-同舟云学术

The genome of HSV-1 translocates through the nuclear pore as a condensed rod-like structure

Published:2006-01-01 Issue:1 Volume:119 Page:23-30
ISSN:1477-9137
Container-title:Journal of Cell Science
language:en
Short-container-title:

Author:

Shahin Victor¹²,Hafezi Wali³⁴,Oberleithner Hans¹,Ludwig Yvonne¹,Windoffer Barbara¹,Schillers Hermann¹,Kühn Joachim E.³⁴

Affiliation:

1. Institute of Physiology II, University of Münster, Robert-Koch-Str. 27b, 48149 Münster, Germany

2. Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1PD, UK

3. Institute of Medical Microbiology, University of Münster, Domagkstr. 10, 48149 Münster, Germany

4. Interdisciplinary Center of Clinical Research (IZKF), Domagkstr. 3, 48149 Münster, Germany

Abstract

Incoming herpes simplex virus type-1 (HSV-1) capsids are known to dock to the nuclear pore complex (NPC) and release their genome. It has remained elusive, however, how the huge viral DNA translocates through the comparatively small NPC channel. In the present study, the interaction of HSV-1 with NPCs was analyzed by atomic force microscopy. In addition to capsids, smaller subviral structures - most with a diameter of 35-40 nm and a length of 130-160 nm - were visualized at the cytoplasmic side of the NPC. These components differed from capsids in their adhesion and stiffness properties, and were the sole subviral structures translocated through dilated NPCs towards the nucleus. It is presumed that they are the HSV-1 genome, and that a change in NPC conformation allows translocation of this genome as a densely packaged, rodlike structure.

Publisher

The Company of Biologists

Subject

Cell Biology

Link

http://journals.biologists.com/jcs/article-pdf/119/1/23/1367245/23.pdf

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