Studies on the Growth of Tissues In Vitro

Abstract

1. Growth of chick periosteal fibroblasts is inhibited by 0.002 M solutions of glyceraldehyde, benzaldehyde, butyl aldehyde, propyl aldehyde and methyl glyoxal. 2. The growth is not inhibited by sodium pyruvate, lactate, propionate, glycerate or benzoate. 3. Sodium pyruvate does not affect the growth under the conditions of the experiments, nor does it alter the inhibition brought about by glyceraldehyde. 4. Growth is inhibited by sodium fluoride and sodium iodoacetate in the concentrations in which these inhibit glucolysis. Growth inhibition does not correspond to inhibition of phosphorylation. 5. Growth is not inhibited by 0.008 M phlorizin. 6. Growth is not immediately inhibited by an atmosphere containing 95% CO and 5% O2. 7. Growth is not immediately inhibited by 0.002 M sodium azide, 0.002 M HCN or by 0.01 M sodium malonate. Azide and cyanide reduce the growth rate after some hours. 8. Growth is not accelerated by the addition of 0.006 M sodium fumarate. 9. Growth is inhibited by 0.01 M sodium ferricyanide. 10. The relationship between carbohydrate metabolism and growth by cell division is discussed in the light of these results.