Laser-mediated osteoblast ablation triggers a pro-osteogenic inflammatory response regulated by reactive oxygen species and glucocorticoid signaling in zebrafish

Author:

Geurtzen Karina123,López-Delgado Alejandra Cristina12ORCID,Duseja Ankita124,Kurzyukova Anastasia125,Knopf Franziska12ORCID

Affiliation:

1. Center for Regenerative Therapies TU Dresden (CRTD), Center for Molecular and Cellular Bioengineering (CMCB), TU Dresden 1 , 01307 Dresden , Germany

2. Center for Healthy Aging, Medical Faculty Carl Gustav Carus, TU Dresden 2 , 01307 Dresden , Germany

3. Laboratory of Clinical and Experimental Endocrinology, Department of Chronic Diseases, Metabolism and Ageing 3 , KU Leuven, 3000 Leuven , Belgium

4. Department of Oncology and Metabolism, Metabolic Bone Centre, Sorby Wing, Northern General Hospital 4 , Sheffield S5 7AU , UK

5. Faculty of Health and Medical Sciences, Biotech Research & Innovation Centre (BRIC), University of Copenhagen 5 , 2200 Copenhagen , Denmark

Abstract

ABSTRACT In zebrafish, transgenic labeling approaches, robust regenerative responses and excellent in vivo imaging conditions enable precise characterization of immune cell behavior in response to injury. Here, we monitored osteoblast-immune cell interactions in bone, a tissue which is particularly difficult to in vivo image in tetrapod species. Ablation of individual osteoblasts leads to recruitment of neutrophils and macrophages in varying numbers, depending on the extent of the initial insult, and initiates generation of cathepsin K+ osteoclasts from macrophages. Osteoblast ablation triggers the production of pro-inflammatory cytokines and reactive oxygen species, which are needed for successful macrophage recruitment. Excess glucocorticoid signaling as it occurs during the stress response inhibits macrophage recruitment, maximum speed and changes the macrophage phenotype. Although osteoblast loss is compensated for within a day by contribution of committed osteoblasts, macrophages continue to populate the region. Their presence is required for osteoblasts to fill the lesion site. Our model enables visualization of bone repair after microlesions at single-cell resolution and demonstrates a pro-osteogenic function of tissue-resident macrophages in non-mammalian vertebrates.

Funder

Deutsche Forschungsgemeinschaft

Technische Universität Dresden

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3