Juvenile hormone downregulates vitellogenin production in Ectatomma tuberculatum (Hymenoptera: Formicidae) sterile workers

Abstract

In the ant Ectatomma tuberculatum (Olivier, 1792), workers have active ovaries and lay trophic eggs that are eaten by the queen and larvae. Vitellogenins are the main proteins found in the eggs of insects and are the source of nutrients for the embryo in the fertilized eggs and for adults when in the trophic eggs. In social insects, vitellogenin titers vary between castes and affect reproductive social status, nursing, foraging, longevity, somatic maintenance, and immunity. In most insects, vitellogenin synthesis is mainly regulated by juvenile hormone. However, in non-reproductive worker ants, this relationship is poorly characterized. This study determined the effects of juvenile hormone on vitellogenin synthesis in non-reproductive E. tuberculatum workers. Juvenile hormone was topically applied onto workers, and the effect on vitellogenin synthesis in the fat body and vitellogenin titers in the haemolymph were analyzed by ELISA and qPCR. Juvenile hormone downregulated protein synthesis and reduced vitellogenin titers in the haemolymph, suggesting that in workers E. tuberculatum, juvenile hormone loses its gonadotrophic function.