Development and persistence of catecholaminergic neurons in cultured explants of fetal murine vagus nerves and bowel

Abstract

Transient catecholaminergic (TC) cells have been found to appear in the vagal pathway and bowel of fetal mice and rats. It has been proposed that these cells are migrating vagal crest-derived precursors of enteric neurons that lose their catecholaminergic properties when they terminally differentiate. In the current experiments, segments of fetal mouse gut were explanted before (day E9) TC cells or any neural markers could be detected in situ. Tyrosine hydroxylase (TH)-immunoreactive neurons developed in vitro in 4/12 such explants; therefore, cells with a catecholaminergic potential are present in the gut of at least some animals prior to the in situ expression of this phenotype. The neurogenic potential of cells in the vagal pathway was similarly tested by studying cultures of explanted vagus nerves (day E11). These studies revealed that neural precursors were present in the vagi and gave rise in vitro to neurons that displayed acetylcholinesterase (AChE) activity and neuron-specific enolase (NSE) immunoreactivity. A subset of these neural precursors were capable of migrating and formed satellite ganglia at a distance from the explants. Coincident expression of NSE and TH immunoreactivities was observed, indicating that at least some of the neurons that developed in vitro were derived from TC cells. Vagal TC cells, therefore, are neurogenic. Catecholaminergic cells did not disappear from cultured explants of vagus nerves or gut provided that these tissues contained TC cells at the time of explantation. Instead, catecholaminergic neurons developed and persisted in vitro for as long as cultures were maintained. These neurons contained aromatic L-amino acid decarboxylase as well as TH, NSE and neurofilament immunoreactivities. In contrast, if the bowel was explanted after the in situ disappearance of TC cells, catecholaminergic cells did not arise in the cultures. These experiments indicate that the period of time during which a catecholaminergic phenotype is expressed by neural precursors in the fetal vagal pathway and gut is not fixed, but can be changed by altering the environment of the cells as occurs when the bowel is grown in vitro; moreover, contact with non-neuronal cells within the bowel is not by itself sufficient to inactivate catecholaminergic expression. The nature of the signal responsible for loss of the catecholaminergic phenotype in situ remains to be determined; however, the persistence of catecholaminergic expression in vitro should facilitate the investigation of this signal.