Transcriptomic landscape of the primitive streak

Author:

Alev Cantas12,Wu Yuping1,Kasukawa Takeya3,Jakt Lars M.4,Ueda Hiroki R.35,Sheng Guojun1

Affiliation:

1. Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.

2. Vascular Regeneration Research Group, Institute of Biomedical Research and Innovation, Kobe, Hyogo 650-0047, Japan.

3. Functional Genomics Unit, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.

4. Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.

5. Laboratory for Systems Biology, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.

Abstract

In birds and mammals, all mesoderm cells are generated from the primitive streak. Nascent mesoderm cells contain unique dorsoventral (D/V) identities according to their relative ingression position along the streak. Molecular mechanisms controlling this initial phase of mesoderm diversification are not well understood. Using the chick model, we generated high-quality transcriptomic datasets of different streak regions and analyzed their molecular heterogeneity. Fifteen percent of expressed genes exhibit differential expression levels, as represented by two major groups (dorsal to ventral and ventral to dorsal). A complete set of transcription factors and many novel genes with strong and region-specific expression were uncovered. Core components of BMP, Wnt and FGF pathways showed little regional difference, whereas their positive and negative regulators exhibited both dorsal-to-ventral and ventral-to-dorsal gradients, suggesting that robust D/V positional information is generated by fine-tuned regulation of key signaling pathways at multiple levels. Overall, our study provides a comprehensive molecular resource for understanding mesoderm diversification in vivo and targeted mesoderm lineage differentiation in vitro.

Publisher

The Company of Biologists

Subject

Developmental Biology,Molecular Biology

Reference64 articles.

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