Affiliation:
1. RUE «Scientific and Practical Center of the National Academy of Sciences of Belarus for Livestock»
Abstract
The concentration of recombinant human lactoferrin (rhLF) in the milk of 22 transgenic goats of different ages was studied: the average concentration of rhLF in the milk of goats-producers of the 4th year of lactation was 13.7% higher than that of goats of the 3rd year of lactation (6.07 and 5.24 g/l, respectively). It was found that the maximum level of recombinant glycoprotein was produced by the mammary gland of animals in the 2nd and 4th quarters of the studied year period. Protein’s concentration in milk measured in the 2nd and 4th quarters was 6.4 g/l and 6.33 g/l for producers of the third year of lactation and 8.14 g/l and 7.02 g/l for goats of the fourth year of lactation, respectively. The correlation between the season of the year and the content of the target protein in milk was detected (p <0.01), it was found that the most active rhLF production in goat milk occurred in late spring and winter periods. Variability in the content of the protein of interest in milk between individuals was noted. During the study a relatively low level of rhLF was established in animal №0747 - 0.11–2.52 g/l, while its maximum value was detected in the producer № 0403 - 4.52–9.06 g/l (more than 4 times difference). The range of individual variability in terms of rhLF content in the milk of goats of the third and the fourth lactation was 0.11–9.06 g/l. Milk of genetically modified goats contains 3 times more lactoferrin than human breast milk. Therefore, rhLF concentration testing in goat milk and tracking the dynamics of this indicator allows us to obtain data that can be used later for breeding pro-grams formation, milk production planning and also to improve the gene pool of transgenic animals.
Publisher
Russian Research Institute of Farm Animal Genetics and Breeding
Reference33 articles.
1. Sorensen M. The proteins in whey / M. Sorensen, S. P. L. Sorensen // Compt. Rend. Trav. Lab. Carlsberg, Ser. Chim. – 1939. – Vol. 23. – P. 35-99.
2. Petrushko E. V., Bogdanovich D. M. Qualitative characteristics of milk from goats-producers of recombinant human lactoferrin in the third and fourth years of lactation // Perspective agrarian and food innovations: materials of the Intern. scientific-practical Conf., Volgograd, June 6-7, 2019 Volgograd, 2019. Part 1. P. 161-171.
3. Bogdanovich D. M., Petrushko E. V. Expression of recombinant human lactoferrin in the milk of goat producers during the year // News of science in the agro-industrial complex. Stavropol: Agrus, 2018. No. 2 (11), vol. 1 (based on the materials of the VI International conference "Innovative developments of young scientists - the development of the agro-industrial complex"). Р. 168-172.4. Masson P. L. An iron-binding protein common to many external secretions / P. L. Masson et al. // Clin. Chem. Acta. – 1966. – Vol. 14. – P. 735-739.
4. Masson P. L. Lactoferrin, an iron binding protein in neutrophilic leukocytes / P. L. Masson et al. // J. Exp. Med. – 1969. – Vol. 130. – P. 643-658.
5. Levay P. F. Lactoferrin: a general review / P. F. Levay et al. // Haematologica. – 1995. – Vol. 80. – P. 252-267.