Hormonal biochemical monitoring at bull-producers of different breeds in the conditions of the Central Black Economic District

Abstract

Purpose: To conduct a comprehensive monitoring of bulls of different breeds in the summer in a comparative perspective to the study of protein-lipid and mineral metabolism, as well as to determine hormonal status on the content of endogenous hormones: testosterone, estradiol, cortisol and serum thyroxine per day taking seed.Materials and methods. For the first time in a Chernozem region of the Russian Federation (Voronezh region) was carried out comprehensive monitoring of bulls of red-motley Holstein (n = 16), Red-and-White (n = 8) and Simmental (n = 6) rocks in the same time of year (summer) of the protein-lipid and mineral metabolism, as well as the level of endogenous hormone (testosterone, estradiol, cortisol, thyroxine) and their cholesterol precursor in comparative aspect, immediately after taking the seed. Age range bulls ranged from 2 to 11 years. General monitoring was conducted on 23 indices of blood serum 3 rocks. The studies were conducted by modern methods, using modern equipment and reagents.Results. At Red Pied Holsteins an increase of total protein 100.45 g / L versus 92 g / l in normal (above the norm of 9.2%, as compared with red and White breed at the level of 11.3%). A similar increase in concentration in the blood of Holstein bulls red-White breed recorded on globulins, 68.87 g / l to 63 g / l in normal (above the norm of 9.3%), and with respect to the red-and-White breed 17, 6%. Also notes the high content of AST bulls red-motley Holstein against the red-and-White 18%, but within the reference range. Condition protein and lipid metabolism in sires only differed in content of creatinine, which amounted to 165.04 micromol / l against 163 mmol / l in normal, 1.6% higher as compared to the red and white breed this index was higher 18.7%. According to the content of testosterone highest rate was observed in bulls red-White breed 54.73 ± 9.51 nmol / l, is 10.2% more than in bulls red checkered-Holstein and 12.45% compared with Simmental breed. Similarly, in terms of serum cortisol - 355.60 ± 39.2 nmol / l in the red-bulls White breed that more than 2-fold compared with bulls Red Pied Holstein and Simmental. The difference is statistically significant at P ≤ 0,01. According to the content of estradiol relatively low levels recorded in the bulls of red-motley breed - 0.265 nmol / L vs. 0.301 nmol / L in red bulls-motley Holstein, and 0.332 nmol / L - Simmental, is lower by 13.6% and 25 , 3%, respectively.Conclusion. It was found that substantially all of the studied parameters in bulls 3 breeds are at reference values, and this gives grounds to assume that all of these breeds are well adapted to the conditions of Black Soil.