Affiliation:
1. Vardhman College of Pharmacy, Koli, Karanja (Lad), Washim, Maharashtra, India
Abstract
Chromatography is a crucial biophysical method that makes it possible to separate, identify, and purify mixture's constituent parts for qualitative and quantitative study. Based on characteristics like size and shape, total charge, the presence of hydrophobic groups on the surface, and capacity to attach to stationary phases, proteins can be purified. Ion exchange, surface adsorption, partition, and size exclusion mechanisms are used in four different separation approaches based on the molecular characteristics and types of interactions. Other chromatographic methods, like column, thin-layer, and paper chromatography, are based on fixed beds. One of the most popular techniques for purifying proteins is column chromatography. Membrane chromatography is largely employed in wastewater treatment applications as well as downstream processes for the separation and purification of proteins and biopolymers. Using a membrane chromatography.