Application of a 3D bioprinter: jet technology for ‘biopatch’ development using cells on hydrogel supports-Reference-Cited by-同舟云学术

Application of a 3D bioprinter: jet technology for ‘biopatch’ development using cells on hydrogel supports

Published:2023-12-12 Issue: Volume: Page:
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

DeMel Derek C¹^ORCID,Wagner Grayson A¹^ORCID,Maresca Jamie A²^ORCID,Geibel John P²³^ORCID

Affiliation:

1. Yale School of Engineering & Applied Science, New Haven, CT 06519, USA

2. John B. Pierce Laboratory, New Haven, CT 06519, USA

3. Department of Surgery & Cellular & Molecular Physiology, Yale University School of Medicine, New Haven, CT 06519, USA

Abstract

Additive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines – HEK 293 (kidney) and K7M2 wt (bone) – were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended periods. Combining a flexible biosupport with application of cells via TIPS printing now for the first time allows for custom cell substrate constructs with various densities to be deployed for regenerative medicine applications.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/btn-2023-0052

Reference20 articles.

1. 3D Bioprinting for Organ Regeneration

2. Tissue Engineering Through 3D Bioprinting to Recreate and Study Bone Disease

3. Nanoengineered Osteoinductive Bioink for 3D Bioprinting Bone Tissue

4. Advances on Bone Substitutes through 3D Bioprinting