Mitochondrial membrane potential probes and the proton gradient: a practical usage guide

Author:

Perry Seth W.12,Norman John P.3,Barbieri Justin4,Brown Edward B.1,Gelbard Harris A.245

Affiliation:

1. Department of Biomedical Engineering, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA

2. Department of Neurology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA

3. Graduate Program in Toxicology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA

4. Center for Neural Development and Disease, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA

5. Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA

Abstract

Fluorescent probes for monitoring mitochondrial membrane potential are frequently used for assessing mitochondrial function, particularly in the context of cell fate determination in biological and biomedical research. However, valid interpretation of results obtained with such probes requires careful consideration of numerous controls, as well as possible effects of non-protonic charges on dye behavior. In this context, we provide an overview of some of the important technical considerations, controls, and parallel complementary assays that can be employed to help ensure appropriate interpretation of results, thus providing a practical usage guide for monitoring mitochondrial membrane potentials with cationic probes. In total, this review will help illustrate both the strengths and potential pitfalls of common mitochondrial membrane potential dyes, and highlight best-usage approaches for their efficacious application in life sciences research.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

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