DEVELOPMENT AND VALIDATION OF CHEMOMETRIC-ASSISTED UV-SPECTROPHOTOMETRIC METHOD FOR EPIGALLOCATECHIN GALLATE AND CURCUMIN IN TABLET FORMULATION

Abstract

A chemometric method, Partial Least Square, was applied for the simultaneous estimation of epigallocatechin gallate and curcumin in tablet formulation. Twenty five mixed sample solutions were prepared for chemometric calibration as training set and sixteen mixed solution for validation set using Full Factorial Design. The absorbance data matrix was obtained by measuring absorbance at 20 different wavelengths in the range of 220 to 410 nm (Δλ = 10 nm). The developed calibration data was used to test tablet formulation containing epigallocatechin gallate and curcumin. The developed methods were validated using RMSECV and RMSEP. The chemometric calculations were performed using Minitab 16.1.1 and Microsoft Excel 2010. The method is also more accurate and precise than conventional UV methods.