Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363-Reference-Cited by-同舟云学术

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Published:2017-09-27 Issue:3 Volume:66 Page:321-326
ISSN:1733-1331
Container-title:Polish Journal of Microbiology
language:
Short-container-title:Pol J Microbiol

Author:

Awadhiya Pushpendra¹,Banerjee Tushar¹,Patil Shridhar¹

Affiliation:

1. Applied Microbiology Laboratory, School of Life Sciences, Devi Ahilya University, Indore, India

Abstract

Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, temperature, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.

Publisher

Polish Society of Microbiologists

Subject

Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology

Link

https://pjm-microbiology.publisherspanel.com/gicid/pdf/01.3001.0010.4858

Reference27 articles.

1. Ahmed S. and B.N. Johri. 1991. A cholesterol degrading bacteria: isolation, characterization and bioconversion. Indian J. Exptl. Biol. 29: 76-77.

2. Asolkar L.V. and Y.R. Chadha. 1979. Diosgenin and Other Steroid Drug Precursors. Publications and Information Directorate, CSIR, New Delhi.

3. Atrat P.G., B. Koch, B. Szekalla and C. Horhold-Schubert. 1992. Application of newly synthesized detergents in the side chain degradation of plant sterols by Mycobacterium fortuitum. J. Basic. Microbiol. 32: 147-157.

4. Banerjee T., A. Shukla, K. Shinde and S. Patil. 2003. Mixed culture bioconversion of 16-dehydropregnenolone acetate to androsta-1,4-diene-3,17-dione: optimization of parameters. Biotechnol. Prog. 19: 662-664.

5. Donova M.V. and O.V. Egorova. 2012. Microbial steroid transformations: current state and prospects. Appl. Microbiol. Biotechnol. 94: 1423-1447.

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