High-throughput particle focusing and separation in split-recombination channel

Abstract

Abstract Inertial microfluidic has been widely applied to manipulate particles or bio-sample based on the inertial lift force and Dean Vortices. This technology provides significant advantages over conventional technologies, including simple structure, high throughput and freedom from an external field. Among many inertial microfluidic systems, the straight microchannel is commonly used to produce inertial focusing, which is a phenomenon that particles or cells are aligned and separated based on their size under the influence of inertial lift force. Besides the inertial lift force, flow drag forces induced by the geometrical structures of microchannel can also affect particle focusing. Herein, a split-recombination microchannel, consisting of curved and straight channels, is proposed to focus and separate particles at high flow rate. As compared with the straight channel, the particle focusing in the split-recombination channel is greatly improved, which results from the combined effects of the inertial lift force, the curvature-induced Dean drag force and the structure of split and recombination. Moreover, the distribution of different-sized particles in designed microchannel is investigated. The results indicate that the proposed microchannel not only enhances the particle focusing but also enables the separation of different-sized particles with high throughput. Finally, it is discovered that the larger length of straight channel and curvature radius of curved channel can result in a more efficient particle separation. Another important feature of designed split-recombination microchannel is that it can be arranged in parallel to handle large-volume samples, holding great potential in lab-on-a-chip applications.