Emergence of dynamic contractile patterns in slime mold confined in a ring geometry

Abstract

Abstract Coordination of cytoplasmic flows on large scales in space and time are at the root of many cellular processes, including growth, migration or division. These flows are driven by organized contractions of the actomyosin cortex. In order to elucidate the basic mechanisms at work in the self-organization of contractile activity, we investigate the dynamic patterns of cortex contraction in true slime mold Physarum polycephalum confined in ring-shaped chambers of controlled geometrical dimensions. We make an exhaustive inventory of the different stable contractile patterns in the absence of migration and growth. We show that the primary frequency of the oscillations is independent of the ring perimeter, while the wavelength scales linearly with it. We discuss the consistence of these results with the existing models, shedding light on the possible feedback mechanisms leading to coordinated contractile activity.