Phytochemical properties and antioxidant activity of wild-grown and cultivated Ganoderma lucidum

Abstract

Abstract The most biologically active compounds of medicinal mushroom Ganoderma lucidum can be classified into polysaccharides and terpenoids. Most of these biological compounds are supposed to associate with its antioxidant activity. Both of wild grown and cultivated G. lucidum have been commercially in demand in Indonesia during the past years. Due to their different growing conditions, the wild-grown and cultivated G. lucidum may contain different levels of effective chemical components which affect their quality and medicinal efficacy. This present study was carried out to determine the differences between wild-grown and cultivated G. lucidum which might be useful in exploring the characteristic of chemical compounds of G. lucidum regarding its antioxidant activity. The physicochemical evaluation was determined using gravimetric method. The phytochemical evaluation includes water – soluble polysaccharides, phenolic, and terpenoids content. The antioxidant activity was evaluated by measuring the radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. Cultivated G. lucidum from Godean has the highest water – soluble polysaccharides (29.86±2.42 GE, mg/g dw) and phenolic content (5.07±0.39 GAE, mg/g dw) among other studied samples. Whereas, cultivated G. lucidum from Gunung Kidul has the lowest water – soluble polysaccharides (21.65±2.45 GE, mg/g dw) and phenolic content (3.21±0.87 GAE, mg/g dw). Both of wild grown G. lucidum have higher terpenoids content compare to all of cultivated G.lucidum. The cultivated – Godean revealed the highest DPPH scavenging activity (the lowest IC50, 344.15±9.57 µg/mL) among of the studied samples. Hence, the results suggested that G. lucidum contained high metabolites compounds and has a potential natural source of antioxidants.