Isolation and Identification of Bird Viruses in Wetland Ecology

Abstract

Abstract In order to study the occurrence of Newcastle disease virus (NDV) in wild birds of Honghu wetland in Hubei, we collected wild bird feces samples in the wild bird habitat of Honghu in Hubei in the winter and spring of 2017 ∼ 2019. By SPF chicken embryo isolation, hemagglutination-positive samples were identified by RT-PCR. A total of 38 NDV and 3 mixed viruses including NDV and avian influenza virus (AIV) were detected. The results showed that the NDV positive rate in wild birds was about 1%. These monitoring results lay the foundation for further understanding the status of NDV carried by wild birds and formulating corresponding preventive measures.