Modification of boron-doped diamond with gold through wet-chemical seeding and electrodeposition techniques for the application of acrylamide biosensor

Abstract

Abstract Acrylamide is known as carcinogenic and neurotoxin substrates. An alternative method for acrylamide detection is by using hemoglobin-based biosensors as it can provide a simple, rapid, and sensitive method. In this research, the electrodes for biosensor of acrylamide were prepared by modifying boron-doped diamond (BDD) with gold and hemoglobin (Hb). Wet-chemical seeding followed by electrochemical overgrowth of seeds technique was performed to modify the BDD electrodes with gold. The characterization with Scanning Electron Microscopy-Energy Dispersive Spectroscopy X-Ray (SEM-EDS) showed the gold coverage of 12.74 % on the BDD surface. The gold-modified BDD was then modified with 0.25 mM Hb. The prepared sensor can be successfully applied in the concentration range of acrylamide from 0.6 to 6 μM with high linearity (R2 = 0.9901) and an estimated limit of detection of 0.845 μM.