Study of a microfluidic chip with converse fluid

Abstract

Abstract The current microfluidic chip design generally forms a stagnation point in the exit flow area of the microfluidic chip. The presence of the stagnation area can cause problems such as choke of channel and sample purity degradation. For this problem, a microfluidic chip with converse fluid was designed. The converse sheath liquid can avoid the adverse effects caused by the presence of the stagnation point. It can prevent the cells from contacting the wall surface, and avoid the blocking problem of cell. At the same time, the introduction of the converse sheath liquid can also focus the sample flow in the sorting channel and the waste channel again, which is convenient for the detection of the sorted sample. The fluid flow state in this microfluidic chip was also simulated, and it verified the benefit of introducing converse sheath fluid, which has reference value for the design of microfluidic chip for cell analysis and sorting.