Improved Bacterial Detection Limit via Wet KOH Etching Pathway Enhanced by Laser

Abstract

Abstract Laser with a fixed wavelength (405nm) at different values of intensities (50, 100, 150, 200 mW/cm2) were used to re synthesis Si nano- pillars as based SERS active substrates. The idea was to form SERS devices with high enhancement factor to sense the low residence of bacteria. The results exposed that the topographical features of the Si nano-pillars layer and so, the performance of SERS sensor could be well- controlled via the adjusting of laser intensity through the laser assisted Wet KOH process. The SERS sensing of low concentrations of shows highest bacterial enhancement factor (E.F = 30.1 × 105) and minimum detection limit (LOD = 3.30 Cfu/ml) were realized from high altitude Si nano- pillars; partly populated with three dimensions AgNPs layer, and the use of 150 mW/cm2 laser power density.