Author:
Bahri S,Sundu B,Aprianto M R
Abstract
Abstract
Coconut flour is one of agriculture waste product, containing high concentration of mannan polysaccharide. This waste product can be used as a substrate to produce mannanase enzyme through fermentation by using inoculum of Aspergilus niger. The aim of the study was to produce mannanase enzyme with high activity from the non-lipid and non-protein coconut flour. The coconut flour was extracted by using 1M NaOH obtain free-protein coconut flour. The non protein coconut flour was then extracted by using hexane to release lipid. The non-protein and non-lipid substrates were then fermented with Aspergilus niger. A completely randomized design was used in this study with various pH (pH 6 7 8 and 9). The result of the study indicated that pH 9 of the substrate produce the highest mannanase activity, being 0.0313 U/ml. In conclusion, optimal activity of mannanase was reached when the pH of substrate was 9.
Subject
General Physics and Astronomy
Cited by
7 articles.
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