Application of atomic absorption spectroscopy method for platinum content determination to study functionalization of bone substitute materials with anticancer drug

Abstract

Abstract Methods of quantitative determination of the platinum content in high-salt solutions have been developed using atomic absorption spectroscopy (AAS). Definitions were carried out for small volume bioassays during the research of functionalization of calcium phosphate substitute materials with anticancer drug, cisplatin. The conditions for the determination of Pt in the flame were optimized. The effects of the cisplatin matrix solutions, salt components and concentrations on the Pt absorption value were found out. The relevance of application of the flame version of the AAS method was shown in order to determine the platinum content in high-salt solutions, over 200 g/l, without matrix separation in a wide range of Pt concentrations from 0.1 to 50 µg/ml. This issue was targeted through the estimation of efficiency of Pt-containing drug incorporation and its dynamics release as an application method in the development of drug delivery systems for bone tumors treatment.