The Detection and Investigation of Tetracycline Resistance Associated Genes Among Shigella Isolates Using Polymerase Chain Reaction and Phylogenetic Analysis Methods In Al-Diwaniyah province, Iraq

Abstract

Abstract Shigellosis is second cause in the world that leading to diarrheal death (164,300 deaths annually, one to three causes that leading to diarrheal deaths in children younger than 5 yeares. Shigella spp are responsible for the illness caused that are enteric bacteria facultative anaerobice, non-motile, Gram-negative rods. almost of 165 millions states of diarrheal yearly, about 163 millions of states is at developing nations. because of the developments of antibiotics resistance over the previous halfe-century, In Shigellosis optiones for Antimicrobial treatments are specific to little numbers of antibiotics overtimes, patterns of antimicrobials resistances in Shigellae spp. has change in order to geographicals positions and treatmentes becames further complicated, The appropirate and active treatments for diseases lead to descrease shed for bacteria and prevents lethale productions. aim of study was to Investigation of The Tetracycline genes in Shigella spp and The Relationship between the Antimicrobial Resistance and Tetracycline genes Using PCR and Phylogenetic tree analysis, Shigella isolates were diagnostic by Biochemical and molecular methods. Shigella isolates were investigated genotypically to detection about harboring Tetracycline Genes includes (tetA), (tetB) by PCR technique. The Shigella isolates showed the high percentage of (tetA) gen about (100%) followed by ,(tetB) (90%). Sequencing of tetA and tetB genes was done also.