Abstract
Abstract
Due to limited intrinsic healing capacity of the meniscus, meniscal injuries pose a significant clinical challenge. The most common method for treatment of damaged meniscal tissues, meniscectomy, leads to improper loading within the knee joint, which can increase the risk of osteoarthritis. Thus, there is a clinical need for the development of constructs for meniscal repair that better replicate meniscal tissue organization to improve load distributions and function over time. Advanced three-dimensional bioprinting technologies such as suspension bath bioprinting provide some key advantages, such as the ability to support the fabrication of complex structures using non-viscous bioinks. In this work, the suspension bath printing process is utilized to print anisotropic constructs with a unique bioink that contains embedded hydrogel fibers that align via shear stresses during printing. Constructs with and without fibers are printed and then cultured for up to 56 d in vitro in a custom clamping system. Printed constructs with fibers demonstrate increased cell and collagen alignment, as well as enhanced tensile moduli when compared to constructs printed without fibers. This work advances the use of biofabrication to develop anisotropic constructs that can be utilized for the repair of meniscal tissue.
Funder
National Science Foundation
National Institutes of Health
Subject
Biomedical Engineering,General Medicine,Biomaterials,Biochemistry,Bioengineering,Biotechnology
Reference92 articles.
1. Meniscal injury: i. Basic science and evaluation;Greis;J. Am. Acad. Orthop. Surg.,2002
2. Current strategies in meniscal regeneration;Hasan;J. Biomed. Mater. Res. B,2014
3. The knee meniscus: structure–function, pathophysiology, current repair techniques, and prospects for regeneration;Makris;Biomaterials,2011
4. The human meniscus: a review of anatomy, function, injury, and advances in treatment;Fox;Clin. Anat.,2015
5. Meniscal repair: the current state and recent advances in augmentation;Bansal;J. Orthop. Res.,2021
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献