Papain Enzyme as Feed Additive for Grouper Cantang (Epinephelus fuscoguttatus-lanceolatus) and Silver Pompano (Triachonus blochii)

Abstract

Abstract This research aims to observe growth and intestines condition of mariculture fish species, grouper cantang dan silver pompano which feed had been incorporated with papain enzyme (PE) containing papaya derived protease. The research was conducted in 2018 at The Floating Net Cages at Pangandaran Beach, Indonesia. A complete randomized design with five treatments and three repetitions was performed. The treatments involved were A (control ) B (1, 2.5% of PE added to feed), C (2.50% of PE added to feed), D (3.75% of PE added to feed), E (5.00% of PE added to feed). In addition, the research experiment if silver pompano with the same manner was conducted in 2020. The treatments were V (2% of PE added to feed), W (3% of PE added to feed), X (4% of PE added to feed), Y (5% of PE added to feed) and Z (6% of PE added to feed) for 2 months of observation time. The results showed that additional PE incorporation to feed could increase Average Daily Gain (ADG) and Feed Convertion Ratio (FCR) on fish. The addition of the papain enzyme dose in cantang grouper feed has a positive effect on the growth and intestinal conditions of the fish. At doses of 3.75% and 5% gave the best response to ADG (2.26 grams/day and 2.30 grams/day), FCR (1.41% and 1.40%) and SR (68% and 69%). The cantang grouper intestine which was given the addition of papain enzyme was 3.75% and 5%, the best conditions were the number of villi (52 and 53 pieces), the best length of the cantang grouper villi was in the 125% and 5% treatments, namely 539.3 and 524.5. Meanwhile the papain enzyme at a dose of 5% gave the best response to body weight, ADG (2.46 gram/day), SGR (0.83 %/day) and SR in silver pompano (100%).