Isolation, Identification and Cultivation of Ganoderma spp. and Evaluation of Their Efficiency in Inhibiting Plant Pathogenic Fungi Fusarium oxysporum and Alternaria tenuissima

Abstract

Abstract Fruit bodies of three Ganoderma isolates coded by Has.AA-6, Has.AA-7 and Has.AA-8 were collected from the trunks of Poplar, Sidr and Apricot trees, respectively, from some districts in Salah al-Din Governorate. It was phenotypically diagnosed to the genus level depending on the shapes, colors and sizes of the fruit bodies as well as the internal tissues and basidiospores. Molecular diagnoses to the species level was carried out depending on the percentage of identical 5.8S rRNA gene sequences with strains of fungi in the World Genetic Bank at the NCBI. Ganoderma isolates identified as G. applanatum Has.AA-6, G. lucidum Has.AA-7 and G. resinaceum Has.AA-8, and recorded in the World Genetic Bank at the NCBI under the accession number ON834523.1, ON834541.1 and ON834527.1, respectively. The results of cultivation of Ganoderma spp. showed a superiority of the biological efficiency of the corn cobs supplemented with wheat bran, which were 27.73, 31.27, 31.64 and 32.07%, compared to unsupplemented this medium, as the biological efficiency reached 26.18, 26.67, 27.21, 27.22, and 27.22% for G. lucidum, G. applanatum, G. resinaceum and G. resinaceum 1049 respectively. The role of the filtrate, mycelium biomass and fruit bodies of Ganoderma spp. against the pathogenic fungi F. oxysporum and A. tenuissima was studied, the results showed that the extracts of all Ganoderma spp. have a significant superiority in inhibiting the growth of both pathogenic fungi compared to the control treatment, the alcoholic extract of G. resinaceum fruit bodies recorded the highest inhibition of the F. oxysporum and A. tenuissima in which the colonies diameters reached 3.21 and 2.4cm, respectively, while G. lucidum gave the lowest colony diameter of F. oxysporum and A. tenuissima, which reached 2.61 and 2.25 cm, respectively, compared to with a diameter of 8.5 cm for colonies of both pathogenic fungi in the control.