Isolation and screening caffeine-degrading bacteria

Abstract

Abstract Naturally, the coffee pulp contains caffeine. Caffeine-degrading bacteria can use caffeine for their growth. The purpose of this research was to obtain high potency of caffeine-degrading bacterial isolates. These bacteria were isolated from naturally-fermented pulp waste of Coffea arabica. The bacteria were isolated by using minimal medium M9 contain 1 g/L caffeine and was screened based on their activity to degrade caffeine. There 13 isolates that successfully isolated by this medium. The research found five isolates had high potency to degrade caffeine. They were KAFS 33, KAFS 34, KAFS 16, KAFS 47, and KAFS 35 respectively. Those isolates were the potential to be further analysis as an agent of decaffeinating coffee.