Effect of water clover (Marsile crenata) extract within tris-fructose citric glycerol extender on frozen semen quality of boer goat

Abstract

Abstract The consistency of the semen extender used is one of the deciding factors in the quality of the frozen semen. Water clover extract (WCE) was supposed to retain the semen content during freeze storage with the antioxidant compounds. The objective of this analysis was to determine the impact on the consistency of post-thawed Boer Goat Semen of the water clover extract supplement. The Boer semen was collected from 3 Boer bucks aged at 3 to 3.5 years Boer bucks which weighed at 40 to 42 kg. The semen was obtained by artificial vagina two days a week and the semen used for this research should have at least 80 percent individual motility and feasibility and maximum anomalies of 10 %. The semen was added with WCE at different concentration levels (0, 2, 4 and 6%) by using Tris-fructose citric glycerol diluents as the base extender. The study was done in a randomized way and the results were evaluated using a variance analysis followed by a multiple range test from Duncan. The results showed that a WCE supplementation with Tris-fructose citric glycerol diluent as the base extensor had a significant (P<.05) effect on motility, viability and integrity of plasma membrane but not on abnormalities (P>0.05). The research shows that the best way to preserve frozen boer goat semen consistency was to add 4% WCE in tris fructose citric glycerol.