Eco Enzyme (EE) as a Novel Approach for Bone Mineral Dissolution: Implications for Organic Fertilizer Production

Abstract

Abstract This study investigates the potential of Eco Enzyme (EE) in dissolving bone minerals (Ca, P, and N) and its implications for organic fertilizer production. EE was produced through a three-month incubation of water, molasses, and organic waste in a 10:1:3 ratio in an airtight container. The characterization of EE involved the analysis of pH, acid and sugar concentration, protease activity, and the enumeration of non-selective bacteria, lactic acid bacteria (LAB), and yeast. Results indicated a higher population of LAB compared to non-selective bacteria and yeast, with final counts of log 7.31 CFU/mL, log 6.88 CFU/mL, and log 6.68 CFU/mL respectively. The pH of EE decreased from 5.7 to 3.45, while acid concentration increased from 0.1% to 2.15%, predominantly lactic acid. Fructose was the most prevalent sugar, decreasing from 4% to 0.9% during fermentation. Protease activity was measured at 15.41 U.mL-1. The study confirmed EE’s capability to dissolve bone minerals, suggesting its potential as a mineral-rich liquid organic fertilizer. This novel approach could provide a sustainable solution for organic waste management and contribute to the global efforts towards sustainable agriculture.