Evaluation of color-based classical conditioning as feeding stimuli in common carp, Cyprinus carpio

Abstract

Abstract This study was conducted to evaluate the application of classical conditioning using light and color as a feeding trigger for common carp (Cyprinus carpio). Common carp (average size 38.8 g, stocking density ten fish/tank) was reared in a 120 l glass aquarium equipped with a top filter, thermostat, and aeration. The designated treatment that used in this study is (1) CTRL treatment whereas color, light position, and feeding position assigned randomly (2) FEED treatment whereas green color was associated with feeding (3) FEEDxSTRESS treatment whereas green color was associated with feeding and red color were associated with stress by giving disturbance in the tank for a short period of time (4) negative control whereas no treatment was given. To evaluate feeding responses, we observe time required for 80% of fish to gather in the feeding area (T80%), the number of fish gather around in the feeding area at 5 seconds after LED that associated with feeding was switched on (DF5s), and total feed intake of the fish. Using two-way ANOVA, the result shows improvement in T80% of FEED treatment compared to initial measurement (178 s vs. 85 s, P=0.012), while no difference was found in DF5s and feed intake.