Identification of galactogogues in Gliricidia maculata

Abstract

Abstract Galactogogues are substances used by periparturient dairy cows for boosting milk production and preventing negative energy balance. A long-term use of commercial galactogogues has a toxic effect to normal health status of both human and animals. The study was conducted to identify herbal galactogogues from bioactive compounds of Gliricidia maculata ethanol extract. The samples were harvested randomly from edible portion of plants at optimal age (80 days). Lyophilization was carried out at 55°C for 3 days followed with pulverization. The bioactive compounds were extracted using ultrasonic assisted extraction method and identified by the GCMS-QP2010S SHIMADZU instrument with Rtx 5 MS column. Spectra produced by MS was translated into chemical structures and data were presented in form of retention time (minutes), peak area (%), molecular formula (CxHyOz), molecular weight and the name of bioactive compound based on the similarity index of the GCMS library. As the result, there were five major galactogogues of Gliricidia maculate namelyphytol, 1-Octadecyne, 2,6,10,15,19,23-hexamethyl-Squalene, n-Eicosane and 4-Hexadecen-6-yne which identified in this study. Since this study was the first work reporting galactogogues derived from Gliricidia maculate, therefore application of its potential could be implemented in the future study of dairy cattle feeding practices.