Evaluation of Thiamine, Pyridoxine and Cobalamin as an Additive to Diluted Goats Semen at Cooling

Abstract

Abstract This study was done in the Animal Field, Department of Animal Production Techniques, Technical Institute of Kufa, Al-Furat Al-Awsat Technical University, to evaluate the effect of adding vitamins (thiamine, pyridoxine, and cobalamin) to improve some goat semen diluent characteristics post-cooling. Five young male goats were used in this study, the experiment was divided into four groups, T1 (control), T2: thiamine (150 microgram (µg)/ 1 ml, T3: Pyridoxine 150 µg/1 ml and T4: Cobalamin (150 µg/1 ml). As the semen was collected and divided into four experimental treatments. The semen was stored for 24, 72 and 120 hours (hr.) at 5 ºC. The study recorded a highly significant rise in sperm motility percentage at 24 hr. by adding 150 µg thiamine, pyridoxine, and cobalamin compared with the T1 (control group) which noticed a high significant reduce during different times of cooling storage. The highest motility recorded after 120 hr. was noticed in group T2 (71.333±1.740) and T3 (70.499±2.027) followed by group T4 (67.833±2.185) than group T1 (control) (62.499±2.185). Non-significant effect of sperm abnormalities after 24 hr. between thiamine, pyridoxine, and cobalamin compared to the control group (T1), however, highly significant differences were shown after 72 hr. of cooling, the group T2 (thiamine) noticed highly significant abnormalities decrease at 72 hr. by 11.200+1.588 and non-significant variations were shown between T1, T3 (Pyridoxine) and T4 (Cobalamin). The results showed a significant decrease in the dead sperm percentage by adding thiamine, pyridoxine, and cobalamin to diluted goat semen after 24 and 72 hr. at cooling compared with the control group which were 18.833 ± 1.201 (T2), 22.167 ± 1.453 (T3), 22.000 ± 1.607 (T4) and 26.500 ± 1.527 (T1 control) for 24 hr. and 21.833 ± 1.443 (T2), 24.833 ± 1.166 (T3), 25.333 ± 2.309 (T4) and 29.500 ± 0.881 (T1 control) for 72 hr. respectively, while no significant differences observed among thiamine, pyridoxine, and cobalamin groups for first and second periods of cooling. After 24 hr., adding thiamine (T2) and cobalamin (T4) observed significantly increasing a high in host % by 76.333±1.453 and 76.167±1.453 respectively against the control group (72.000±0.763). Non-significant variations were noticed among thiamine (T2), and cobalamin (T4) for all periods of cooling. It could be concluded that adding the vitamins Thiamine, Pyridoxine, and Cobalamin at cooling periods have a positive role in enhancing the characteristics of cooled goat semen.