The state of polymorphism of genes affecting the meat quality in micropopulations of meat simmentals

Abstract

Abstract A comparative study of the biological characteristics of the heifers Bredy meat type of two populations located in Russia and Kazakhstan was carried out to determine the state of genes associated with the meat quality in the genotypeCAPN1, GDF5, TG5, BGL. In genotyping, we used the real-time PCR method using oligonucleotide primers (Gen Bank), for each primer individually in the corresponding sequences. Studies for improve breeding by innovative methods revealed the frequencies of genotypes and alleles for marker genes CAPN1, TG5, BGL and GDF5, which are related to meat quality indicators, a certain displacement in the genotypes of the compared populations of the Bredy meat type of Kazakhstan and Russia according to the CAPN1 gene (χ2 = 8.52) and the TG5 gene (χ2 = 4.73). The genotyping result of animals from LLC “Breeding plant Bredy” were analyzed from the point of view of attributing carriers of different genotypes to the offspring of bulls-producers Fakir, Kust and Chizhik, in order to determine the producer giving offspring with predictably high nutritional and biotechnological properties of beef. A comparison of the carriers of the identified genotypes of the studied genes by the characteristics used in the evaluation of young animals by their own productivity did not give significant results, however, showed trends by which scientific research can be directed. An assessment of the bulls-producers of the successors of the different line of meat simmentals on the quality of heifers – daughters showed that the largest comprehensive index was set for the bull-producer Bush 39046 – 102.7, which was recognized as an improver. The assignment of all, without exception, heifers of different groups, evaluated bulls-producers, to the highest class elite-record, indicates the value of all the studied genotypes.