Effect of Alcoholic Extract of Syntrichia intermedia on some Yeasts Isolated from Mobile Phones and the User’s Finger

Abstract

Abstract Syntrichia intermedia was isolated from the Amadiya region in northern Iraq for the first time, as it was used against the yeast Brettanomyces bruxellensis, Candida krusei and the yeast Meyerozyma caribbica isolated fore the first one and registered as a new strain in the Gen Bank of the National Center for Biotechnology Information (NCBI) with identification number LC723825.1. Three methods were used to determine the effect of the alcoholic extract of S. intermedia at the following concentrations, 1, 2, 4, 6, 8, and 10 mg/ml against yeast isolates, When using the first method-turbidity measurement the concentrations were shown starting from 1 mg/ml an inhibitory effect respectively up to the highest concentration of 10 mg/ml against yeast B. bruxellensis, C. krusei and M. caribbica. and When using the second method-disc diffusion the following concentration showed the highest inhibition percentage the concentration of 2 mg / ml with a diameter of (12.50 mm) against the yeast B. bruxellensis. and The concentrations 1 with a diameter of (11.0 mm) against the yeast C. krusei and The concentrations 4 mg / ml with a diameter of (11.50 mm) Compared with the antibiotic Nystatin which showed a higher percentage of inhibition than the alcoholic extract, While the third method-diffusion by wells was used and the concentration of 4 mg / ml showed the highest percentage of inhibition with a diameter of (23.0 mm) for yeast B. bruxellensis and a concentration of 6 mg / ml showed the highest percentage of inhibition with a diameter of (19.00 mm) against yeast C. krusei. and a concentration of 8 mg / ml showed the highest percentage of inhibition with a diameter of (15.67 mm) against yeast M. caribbica compared with the antibiotic Nystatin, which showed less inhibition than the alcoholic extract, so the yeast B. bruxellensis was more sensitive to the alcoholic extract, followed by the yeast M. caribbica. Then the yeast When using the method of diffusion by discs and digging, either by measuring the turbidity the yeast M. caribbica was the most sensitive to the alcoholic extract followed by C. krusei Then the yeast B. bruxellensis, so the turbidity measurement method is considered one of the best methods that can be used to measure the effect of the alcoholic extract because of measuring the turbidity using a spectrophotometer, followed by the diffusion method by wells, then the method of diffusion by discs.