Identification of some Phenolic Compounds of Purslane Extracts (Portulaca Oleraceae L.) and Evaluation its Content from vit.E and vit.C and its Antioxidant Activity in Iraq

Abstract

Abstract The phenolic compounds present in the four extracts of both stems and leaves of the Portulaca Oleraceae L. were diagnosed using HPLC, and the following observations were made: In the alcoholic extract of the leaves, the highest concentration was found in Gallic acid at 77.89 ppm, followed by other phenolic compounds represented by Quercetin, chlorogenic acid, Ferulic acid, Kemopferol, and p-Coumaric at concentrations of 55.8, 50.22, 40.25, 24.15, and 19.25 ppm, respectively. In the aqueous extract of the leaves, the highest concentration was found in Gallic acid at 62.59 ppm, followed by other phenolic compounds Quercetin, chlorogenic acid, Ferulic acid, p-Coumaric, and Kemopferol at concentrations of 41.25, 40.23, 29.58, 16.25, and 16.22 ppm, respectively. In the alcoholic extract of the stems, it was found to contain four phenolic compounds with Gallic acid having the highest concentration at 40.56 ppm, followed by chlorogenic acid, Quercetin, and Kemopferol at concentrations of 33.65, 30.25, and 14.25 ppm, respectively. In the aqueous extract of the stems, it was found to contain four phenolic compounds with Gallic acid having the highest concentration at 33.69 ppm, followed by Quercetin, chlorogenic acid, and Kemopferol at concentrations of 21.45, 20.58, and 11.48 ppm, respectively. The vitamin C and vitamin E contents were evaluated in the extracts. The highest concentration of vitamin E was found in the aqueous extract of the leaves, followed by the alcoholic extract of the leaves, the aqueous extract of the stems, and the alcoholic extract of the stems, with concentrations of 54.1, 33.5, 31.5, and 25.9 ppm, respectively. As for vitamin C concentration, it was found to be highest in the aqueous extract of the leaves, followed by the aqueous extract of the stems, the alcoholic extract of the leaves, and finally the alcoholic extract of the stems, with concentrations of 85.6, 66.5, 52.8, and 35.6 ppm, respectively. The antioxidant activity of the aqueous and alcoholic extracts of the leaves and stems of Portulaca Oleraceae L. was measured by assessing the removal percentage using the FeCl2 and H2O2 methods. The alcoholic extract of the leaves showed the highest removal percentage of 94.73% at a concentration of 150 mg/ml using the FeCl2 method, and the highest DNA protection percentage of 92.73% at a concentration of 150 mg/ml using the H2O2 method.