Healing of diabetic ulcers using jamaican cherry leaf extract cream (Muntingia calabura l.) based on collagen density in white rats (Rattus norvegicus)

Abstract

Abstract This research aims to determine the potential of jamaican cherry leaf extract cream (JCLEC) to accelerate the healing process of diabetic ulcers in white rat based on collagen density. The research used 4 month old male white rats (Rattus norvegicus) which were divided into K0 (negative control), KP (silver sulfadiazine), K1 (5% JCLEC), K2 (10% JCLEC), and K3 (15% JCLEC). An incision was made on the back of the rat then given JCLEC. Days 3, 7, and 14 after treatment, the wound tissue was collected after the rat were euthanized. Skin tissue was processed and stained with picrosirious red. The rate of wound healing was observed. Collagen density was presented in scoring. Based on the treatment group and observation time, there was a significant difference in collagen density between the treatment groups compared to the control (P<0.05). Based on the treatment group, the scoring of the collagen density was 1.78±0.6 (K0); 2.56±0.88 (KP); 1.78±0.4 (K1); 2.56±0.88 (K2); and 3.1±0.78 (K3). Based on observation time, the average collagen density scoring was 1.8±0.3 (D-3); 2.6±0.4 (D-7); and 2.8±0.6 (D-14). The conclusion is the 15% JCLEC has the potential to accelerate the healing of diabetic ulcers in white rats by stimulate collagen density.