The Pregnancy Evaluation on Ongole Crossbred Cows by using Liquid Semen and Frozen Semen

Abstract

Abstract Artificial Insemination using liquid semen is needed for areas where there is no liquid nitrogen and the high price of liquid Nitrogen and equipment for storing frozen semen. The purpose of this study was to determine the success rate of Artificial Insemination using Frozen semen and liquid semen on different diluents in cattle Ongole crossbred. The research method used was Field experiment design. The material used was 81 Ongole Crossbred cows. Each treatment was 27 head. Control was T0 = 27 Cows in AI use frozen semen, T1 = 27 Cows in AI uses Liquid semen with CEP-3 + 10% egg Yok diluter and T2 = 27 Cows in AI use Liquid semen with Tris Amino methan + 20% egg Yok diluter. Cows were selected by purposive sampling which was normal reproduction and showing normal signs of estrus. AI methods was Recto vaginal method with 4+ semen deposition (Uterus Position). The parameter observed were Non Return Rate and Conception rate. The pregnancy determination using the per rectal palpation method. The results showed that the value of NRR-1, NRR-2 and NRR-3 in T0 = 81,48%, 81,48% and 81,48%; of T1 = 81,48%, 81,48% and 70,37% while T2 = 96,30%, 88,89% and 81,48%. Whereas the Conception rate was at T0,T1 and T2 = 62,96%, 51,85% dan 59,26%. In conclusion, the success rate of liquid semen AI using CEP-3 + 20% egg yolk diluent and Tris Aminomethan + 20% egg yolk gave the same effect compared to AI using frozen semen.