Evidence on temperature and concentration of reducing agents to control the nanoparticles growth and their microbial inhibitory efficacy

Abstract

Abstract The present study deals with the nanoparticles synthesis from Impatiens balsamina L. plant flower extract. The concentration of reducing agent (flower extract) and different temperature is involved in the reaction. Sixteen different silver nanoparticles were synthesized with using different ratios of the reaction mixture and different temperature. The different silver nanoparticles are different color based on the temperature and concentration of reaction mixture. The synthesized silver nanoparticles are characterized by UV/vis Spectrophotometer. The microorganisms Bacillus subtilis are highly inhibited by (90:10/60 °C; 99:1/70 °C; 92:2/70 °C; and 90:10/70 °C) silver nanoparticles. Pseudomonas aeruginosa are highly inhibited by 98:2/50 °C and 90:10/70 °C based silver nanoparticles. The bacterial species Staphylococcus aureus are highly inhibited by 98:2/50 °C and 98:2/70 °C. The E. coli was inhibited by 99:1/70 °C; 98:2/70 °C and 90:10/70 °C based silver nanoparticles. The temperature and concentration of reducing agents can play a significant role in controlling the growth of nanoparticles. This study is one of the evidence on temperature and concentration of reducing agents to control the nanoparticles growth and their microbial inhibitory efficacy.