Dynamic context-dependent regulation of auxin feedback signaling in synthetic gene circuits

Author:

Avdovic Merisa1,Garcia-Navarrete Mario1,Ruiz-Sanchis Diego1ORCID,Wabnik Krzysztof1ORCID

Affiliation:

1. Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación Agraria y Alimentaria (INIA/CSIC), 28223 Madrid, Spain

Abstract

Phytohormone auxin plays a key role in regulating plant organogenesis. However, understanding the complex feedback signaling network that involves at least 29 proteins in Arabidopsis in the dynamic context remains a significant challenge. To address this, we transplanted an auxin-responsive feedback circuit responsible for plant organogenesis into yeast. By generating dynamic microfluidic conditions controlling gene expression, protein degradation, and binding affinity of auxin response factors to DNA, we illuminate feedback signal processing principles in hormone-driven gene expression. In particular, we recorded the regulatory mode shift between stimuli counting and rapid signal integration that is context-dependent. Overall, our study offers mechanistic insights into dynamic auxin response interplay trackable by synthetic gene circuits, thereby offering instructions for engineering plant architecture.

Funder

Comunidad de Madrid

Ministerio de Ciencia, Innovación y Universidades

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

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