Author:
Tatara Alexander M.,Koons Gerry L.,Watson Emma,Piepergerdes Trenton C.,Shah Sarita R.,Smith Brandon T.,Shum Jonathan,Melville James C.,Hanna Issa A.,Demian Nagi,Ho Tang,Ratcliffe Anthony,van den Beucken Jeroen J. J. P.,Jansen John A.,Wong Mark E.,Mikos Antonios G.
Abstract
Large mandibular defects are clinically challenging to reconstruct due to the complex anatomy of the jaw and the limited availability of appropriate tissue for repair. We envision leveraging current advances in fabrication and biomaterials to create implantable devices that generate bone within the patients themselves suitable for their own specific anatomical pathology. The in vivo bioreactor strategy facilitates the generation of large autologous vascularized bony tissue of customized geometry without the addition of exogenous growth factors or cells. To translate this technology, we investigated its success in reconstructing a mandibular defect of physiologically relevant size in sheep. We fabricated and implanted 3D-printed in vivo bioreactors against rib periosteum and utilized biomaterial-based space maintenance to preserve the native anatomical mandibular structure in the defect site before reconstruction. Nine weeks after bioreactor implantation, the ovine mandibles were repaired with the autologous bony tissue generated from the in vivo bioreactors. We evaluated tissues generated in bioreactors by radiographic, histological, mechanical, and biomolecular assays and repaired mandibles by radiographic and histological assays. Biomaterial-aided mandibular reconstruction was successful in a large superior marginal defect in five of six (83%) sheep. Given that these studies utilized clinically available biomaterials, such as bone cement and ceramic particles, this strategy is designed for rapid human translation to improve outcomes in patients with large mandibular defects.
Funder
HHS | National Institutes of Health
DOD | United States Army | MEDCOM | Armed Forces Institute of Regenerative Medicine
Robert and Janice McNair Foundation
HHS | NIH | National Institute of Dental and Craniofacial Research
HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases
Publisher
Proceedings of the National Academy of Sciences
Cited by
45 articles.
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