Author:
Goff Loyal A.,Groff Abigail F.,Sauvageau Martin,Trayes-Gibson Zachary,Sanchez-Gomez Diana B.,Morse Michael,Martin Ryan D.,Elcavage Lara E.,Liapis Stephen C.,Gonzalez-Celeiro Meryem,Plana Olivia,Li Eric,Gerhardinger Chiara,Tomassy Giulio S.,Arlotta Paola,Rinn John L.
Abstract
Long noncoding RNAs (lncRNAs) have been implicated in numerous cellular processes including brain development. However, the in vivo expression dynamics and molecular pathways regulated by these loci are not well understood. Here, we leveraged a cohort of 13 lncRNA-null mutant mouse models to investigate the spatiotemporal expression of lncRNAs in the developing and adult brain and the transcriptome alterations resulting from the loss of these lncRNA loci. We show that several lncRNAs are differentially expressed both in time and space, with some presenting highly restricted expression in only selected brain regions. We further demonstrate altered regulation of genes for a large variety of cellular pathways and processes upon deletion of the lncRNA loci. Finally, we found that 4 of the 13 lncRNAs significantly affect the expression of several neighboring protein-coding genes in a cis-like manner. By providing insight into the endogenous expression patterns and the transcriptional perturbations caused by deletion of the lncRNA locus in the developing and postnatal mammalian brain, these data provide a resource to facilitate future examination of the specific functional relevance of these genes in neural development, brain function, and disease.
Funder
HHS | NIH | National Institute of Mental Health
HHS | NIH | National Institute of Neurological Disorders and Stroke
Publisher
Proceedings of the National Academy of Sciences
Cited by
145 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献