Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels-Reference-Cited by-同舟云学术

Histone methylation-mediated microRNA-32-5p down-regulation in sensory neurons regulates pain behaviors via targeting Cav3.2 channels

Published:2022-03-30 Issue:14 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Qi Renfei¹²^ORCID,Cao Junping¹²³^ORCID,Sun Yufang¹²⁴,Li Yanping¹²,Huang Zitong¹²,Jiang Dongsheng⁵^ORCID,Jiang Xing-Hong¹²,Snutch Terrance P.⁶⁷^ORCID,Zhang Yuan⁸⁹^ORCID,Tao Jin¹²⁴^ORCID

Affiliation:

1. Department of Physiology and Neurobiology, Medical College of Soochow University, Suzhou 215123, People’s Republic of China

2. Centre for Ion Channelopathy, Medical College of Soochow University, Suzhou 215123, People’s Republic of China

3. Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou 221004, People’s Republic of China

4. Jiangsu Key Laboratory of Neuropsychiatric Diseases, Soochow University, Suzhou 215123, People’s Republic of China

5. Institute of Regenerative Biology and Medicine, Helmholtz Zentrum München, München 81377, Germany

6. Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada

7. Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, BC V6T 1Z4, Canada

8. Department of Geriatrics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, People’s Republic of China

9. Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou 215004, People’s Republic of China

Abstract

Significance In this study, we identify microRNA-32-5p (miR-32-5p) as a key functional noncoding RNA in trigeminal-mediated neuropathic pain. We report that injury-induced histone methylation attenuates the binding of glucocorticoid receptor to the promoter region of the miR-32-5p gene and decreases the expression of miR-32-5p, in turn promoting the development of neuropathic pain through regulation of Cav3.2 channels. miRNA-mediated gene regulation has been proposed as a therapeutic approach in neuropathic pain. Our findings identify miR-32-5p replenishment as a therapeutic strategy for treating chronic neuropathic pain.

Funder

National Natural Science Foundation of China

National Science Foundation of Colleges and Universities in Jiangsu Province

Natural Science Foundation of Jiangsu Province

Research Innovation Program for College Graduates of Jiangsu Province

Science and Technology Bureau of Suzhou

Jiangsu Key Laboratory of Neuropsychiatric Diseases

Priority Academic Program Development of Jiangsu Higher Education Institutions

Gouvernement du Canada | Canadian Institutes of Health Research

Publisher

Proceedings of the National Academy of Sciences

Subject